29,106 research outputs found
Nondeterministic Instance Complexity and Proof Systems with Advice
Motivated by strong Karp-Lipton collapse results in bounded arithmetic, Cook and KrajĂÄek [1] have recently introduced the notion of propositional proof systems with advice. In this paper we investigate the following question: Given a language L , do there exist polynomially bounded proof systems with advice for L ? Depending on the complexity of the underlying language L and the amount and type of the advice used by the proof system, we obtain different characterizations for this problem. In particular, we show that the above question is tightly linked with the question whether L has small nondeterministic instance complexity
Different populations of RNA polymerase II in living mammalian cells
RNA polymerase II is responsible for transcription of most eukaryotic genes, but, despite exhaustive analysis, little is known about how it transcribes natural templates in vivo. We studied polymerase dynamics in living Chinese hamster ovary cells using an established line that expresses the largest (catalytic) subunit of the polymerase (RPB1) tagged with the green fluorescent protein (GFP). Genetic complementation has shown this tagged polymerase to be fully functional. Fluorescence loss in photobleaching (FLIP) reveals the existence of at least three kinetic populations of tagged polymerase: a large rapidly-exchanging population, a small fraction resistant to 5,6-dichloro-1-ÎČ-D-ribofuranosylbenzimidazole (DRB) but sensitive to a different inhibitor of transcription (i.e. heat shock), and a third fraction sensitive to both inhibitors. Quantitative immunoblotting shows the largest fraction to be the inactive hypophosphorylated form of the polymerase (i.e. IIA). Results are consistent with the second (DRB-insensitive but heat-shock-sensitive) fraction being bound but not engaged, while the third (sensitive to both DRB and heat shock) is the elongating hyperphosphorylated form (i.e. IIO)
Advective relief of CO2 limitation in microphytobenthos in highly productive sandy sediments
Following field observations of increased photosynthesis at increased rates of sediment flushing in sandy sediments, we conducted a series of laboratory experiments to elucidate the mechanism behind these observations. Column experiments in which water was pumped though sand at rates ranging from 0 to 613 L mâ2 dâ1 showed that carbon (C) fixation, as measured using carbonâ14 (14C) incorporation, increased from 6.4 to 8.6 mmol mâ2 hâ1 with increasing rates of flushing. Bottle incubations showed that the addition of inorganic nutrients [ammonium ion (NH4+), inorganic phosphate (HPO4â), silicic acid Si(OH)4] did not stimulate C fixation over shortâterm incubations. Microprofiles of pH showed that the pH within the photic zone increased to 8.9, reducing free carbon dioxide (CO2) concentrations to ~0.5 ”mol Lâ1. Further bottle incubations, where pH and total inorganic carbon (TCO2) were manipulated, showed that high pH (9.6) did not affect photosynthesis if free CO2 was present at concentrations of 10 ”mol Lâ1, suggesting a direct effect of low free CO2 concentrations. 14C fixation profiles at a resolution of 100 ”m recorded by bâradiation imaging showed that while the depth specific maximum rates of C fixation were the same under both diffusive and advective (flushed) conditions, the integrated rates of photosynthesis were highest under flushed conditions because of a thickening of the photosynthetic zone. We conclude that advective poreâwater transport can enhance benthic photosynthesis in shallow permeable sand sediments by counteracting CO2 limitation
Muscle protein and glycogen responses to recovery from hypogravity and unloading by tail-cast suspension
Previous studies in this laboratory using the tail-bast hindlimb suspension model have shown that there are specific changes in protein and carbohydrate metabolism in the soleus muscle due to unloading. For example, 6 days of unloading caused a 27% decrease in mass and a 60% increase in glycogen content in the soleus muscle, while the extensor digitorum longus muscle was unaffected. Also, fresh tissue tyrosine and its in vitro release from the muscle are increased in the unloaded soleus, indicating that this condition causes a more negative protein balance. With these results in mind, studies to investigate the effect of hypogravity on protein and carbohydrate metabolism in a number of rat hindlimb muscles were carried out
Quasi-circular Orbits for Spinning Binary Black Holes
Using an effective potential method we examine binary black holes where the
individual holes carry spin. We trace out sequences of quasi-circular orbits
and locate the innermost stable circular orbit as a function of spin. At large
separations, the sequences of quasi-circular orbits match well with
post-Newtonian expansions, although a clear signature of the simplifying
assumption of conformal flatness is seen. The position of the ISCO is found to
be strongly dependent on the magnitude of the spin on each black hole. At close
separations of the holes, the effective potential method breaks down. In all
cases where an ISCO could be determined, we found that an apparent horizon
encompassing both holes forms for separations well inside the ISCO.
Nevertheless, we argue that the formation of a common horizon is still
associated with the breakdown of the effective potential method.Comment: 13 pages, 10 figures, submitted to PR
Privacy Mining from IoT-based Smart Homes
Recently, a wide range of smart devices are deployed in a variety of
environments to improve the quality of human life. One of the important
IoT-based applications is smart homes for healthcare, especially for elders.
IoT-based smart homes enable elders' health to be properly monitored and taken
care of. However, elders' privacy might be disclosed from smart homes due to
non-fully protected network communication or other reasons. To demonstrate how
serious this issue is, we introduce in this paper a Privacy Mining Approach
(PMA) to mine privacy from smart homes by conducting a series of deductions and
analyses on sensor datasets generated by smart homes. The experimental results
demonstrate that PMA is able to deduce a global sensor topology for a smart
home and disclose elders' privacy in terms of their house layouts.Comment: This paper, which has 11 pages and 7 figures, has been accepted BWCCA
2018 on 13th August 201
A Search for Stellar Obscuration Events due to Dark Clouds
The recent detections of a large population of faint submillimetre sources,
an excess halo gamma-ray background, and the extreme scattering events observed
for extragalactic radio sources have been explained as being due to baryonic
dark matter in the form of small, dark, gas clouds. In this paper we present
the results of a search for the transient stellar obscurations such clouds are
expected to cause. We examine the Macho project light curves of 48 x 10^6 stars
toward the Galactic bulge, LMC and SMC for the presence of dark cloud
extinction events. We find no evidence for the existence of a population of
dark gas clouds with Av > 0.2 and masses between ~ 10^-4 and 10^-2 M_solar in
the Galactic disk or halo. However, it is possible that such dark cloud
populations could exist if they are clustered in regions away from the observed
lines of sight.Comment: 13 pages, 9 figures, submitted to Ap
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